BBWV, PNRSV and SLRSV tested by PCR from 2025 onwards

Publicationdate: 17 december 2024

From 1 January 2025, we will change our virus testing methods for Broad bean wilt virus 1 and 2 (BBWV1/2), Prunus necrotic ringspot virus (PNRSV) and Strawberry latent ringspot virus (SLRSV). We will conduct these tests from now on using the PCR method. With PCR, we are well able to cover the wide variation in isolates.

The real-time PCR is a technique to rapidly propagate DNA

This is done using pathogen-specific building blocks. The increase in DNA can be digitally monitored at any time. This makes it possible to confirm faster and with more certainty whether the pathogen is actually present in (suspected) plant material.

Viruses

SLRSV

This virus causes problems in the northern hemisphere in e.g. outdoor crops. In several countries, it is considered a quarantine organism. The virus is transmitted by nematodes and can be seed-borne in some crops. If crops are vegetatively propagated, the virus remains present.

BBWV1/2

The viruses BBWV1 and 2, occur worldwide in a wide range of crops. These viruses are spread by multiple species of aphids.

PNRSV

PNRSV can cause chlorotic spotting/circulation and hail shot in Prunus species, among others, but also cause symptoms in rose (Rosa spp., one of the causal agents of rose mosaic) and hops (Humulus lupulus). PNRSV is found worldwide because of its easy transmission through plant propagation methods and infected seed.

BBWV, PNRSV and SLRSV on plant material

Sample: maximum 2, 10 or 25 leaf(pieces)

Fee test: € 9,20, € 17,70 and € 48,90 respectively

Duration of test: 4 days

For basic amounts see price list Laboratories.

Naktuinbouw Laboratories support sector

To support the sector, Naktuinbouw Laboratories continues to develop new tests. Those tests can support diagnoses on plants with symptoms or just checking for and guaranteeing the absence of certain plant pathogens.

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